FAQ

Heano matanho akajairwa ekushandisa muchina wecentrifuge:

1. Zvijaire nemuchina chaiwo wecentrifuge wauchange uchishandisa. Verenga mirairo yemugadziri uye ziva nezvezvikamu zvakasiyana uye marongero.

2. Ita shuwa kuti muchina wecentrifuge uri muchimiro chakanaka chekushanda uye wakarongedzwa zvakanaka kune chaiyo application yauchange uchiishandisa.

3. Takura sampuli mu centrifuge rotor. Iwo masampuli anofanirwa kugoverwa zvakaenzana uye akaenzana mu rotor kudzivirira kukuvadzwa kwemuchina.

4. Vhara chivharo chemushini we centrifuge zvakachengeteka. Muchina haushande kunze kwekunge chivharo chakanyatsovharwa.

5. Isa kukurumidza kwaunoda uye nguva yemuchina we centrifuge. Iwo chaiwo marongero anoenderana nerudzi rwemasampuli ari kugadziriswa uye protocol yauri kutevera.

6. Tanga muchina wecentrifuge. Iva nechokwadi chekumira wakajeka pamushini panguva yekushanda kuti udzivise kukuvara kubva pakufamba zvikamu.

7. Kamwe centrifuge run yapera, nyatsobvisa sampuli kubva kune rotor. Iva nechokwadi chekubata sampuli zvinyoro uye kudzivirira kukanganisa chero zvidimbu zvingave zvakaumba panguva ye centrifugation process.

8. Rasa chero zvinhu zvakaraswa zvinoenderana neakakodzera maprotocol uye chenesa muchina wecentrifuge zvakakwana usati washandisa uye mushure mekushandisa.

Zvakakosha kuziva kuti michina yecentrifuge inogona kuva nengozi kana ikasashandiswa nemazvo. Gara uchitevera mirairo yekuchengetedza uye gamuchira kudzidziswa kwakakodzera usati washandisa muchina wecentrifuge.

Centrifugation inzira yakajairika inoshandiswa kupatsanura zvikamu zvakasiyana zveropa muhomwe yeropa.

Heano maitiro akajairwa anobatanidzwa mucentrifuging bhegi reropa:

1. Iva nechokwadi chokuti bhegi reropa rakanyorwa zvakanaka nemashoko omurwere uye rudzi rwechigadzirwa cheropa chiri mukati.

2. Isa bhegi reropa mumushini wecentrifuge. Muchina wacho unofanira kugadzirwa maererano nemirayiro yomugadziri wacho woenzaniswa nokuda kworudzi chairwo rwechigadzirwa cheropa chiri kugadzirwa.

3. Vhara chivharo chemushini we centrifuge uye tanga kutenderera. Iko kumhanya uye nguva yekutenderera kuchaenderana nerudzi rwechigadzirwa cheropa chiri kugadziriswa.

4. Sezvo bhegi reropa rinotenderera, zvikamu zvakasiyana-siyana zveropa zvichaparadzana zvichienderana nekuwanda kwavo. Masero matsvuku eropa anorema anogara kuzasi kwebhegi, nepo plasma yakareruka ichikwira kumusoro.

5. Kana kutenderera kwapera, nyatsobvisa bhegi reropa kubva mumushini we centrifuge. Iva nechokwadi chekubata bhegi zvinyoro kuti urege kuvhiringidza zvikamu zvakaparadzana.

6. Zvikamu zvakapatsanurwa zvino zvinogona kutorwa kubva mubhegi reropa uchishandisa sterile transfer set. Masero matsvuku eropa anogona kushandiswa kuisirwa ropa, nepo plasma inogona kushandiswa kune zvimwe zvinangwa zvekurapa.

Zvakakosha kuziva kuti kudzidziswa kwakakodzera uye nzira dzekuchengetedza dzinofanira kutorwa pakubata zvinhu zvinogadzirwa neropa uye kushandisa michina yecentrifuge.

Kumhanya kunodiwa kupatsanura ropa uchishandisa centrifuge kunoenderana nerudzi rweropa raunoda kupatsanura uye rudzi rwecentrifuge riri kushandiswa. Kazhinji, pazvinangwa zvekiriniki zvenguva dzose, kukurumidza kukurumidza kwecentrifugation kunoshandiswa kupatsanura ropa kuri pakati pe3000 kusvika 4000 revolutions paminiti (RPM). Kumhanya uku kunokwana kuparadzanisa ropa muzvikamu zvaro, zvinoti masero matsvuku eropa, masero machena eropa, uye plasma.

Nekudaro, kana iwe uchida kupatsanurwa kwakanyanya kwezvikamu zveropa, sekuparadzanisa mamwe marudzi emasero kana zvimedu, ungangoda kugadzirisa centrifugation kumhanya zvinoenderana. Semuenzaniso, kuparadzanisa maplatelet kubva kuplasma, kasi ye centrifugation inogona kuwedzera kusvika ku4000-5000 RPM.

Zvakakosha kuziva kuti iyo yakakodzera centrifugation speed inogona kusiyana zvichienderana nerudzi rwe centrifuge, rudzi rwechubhu inoshandiswa, uye huwandu hwemuenzaniso uri centrifuged.

Naizvozvo, zvinokurudzirwa kutevedzera iwo chaiwo mirairo kune yako chaiyo centrifuge uye chubhu mhando kuti uwane yakakwana mhedzisiro.

Kuenzanisa centrifuge kwakakosha kuti ive nechokwadi chekuti masampuli akagoverwa zvakaenzana panguva yekutenderera, izvo zvinogona kubatsira kudzivirira kukuvadzwa kweiyo centrifuge uye kugadzirisa iko kurongeka uye kuberekazve kwemhedzisiro.

Heano maitiro akajairwa ekuenzanisa centrifuge:

1. Tarisa huwandu hwekutakura mutoro we centrifuge uye usapfuure.

2. Isa machubhu kana zvibatiso zvemuenzaniso mu centrifuge rotor mu symmetrical pattern yakapoteredza axis yepakati ye rotor.

3. Kana zvichidikanwa, wedzera machubhu asina chinhu kana kuenzanisa zviyero kune nzvimbo dzisina chinhu mu rotor kuti uwane kuenzana.

4. Vhara chivharo che centrifuge uye uone kuti yakachengetedzwa zvakanaka.

5. Isa iyo centrifuge kune yaunoda kumhanya uye uimhanyisa kwenguva pfupi.

6. Misa centrifuge uye tarisa chero kuzununguka kana kudengenyeka. Kana centrifuge isina kuenzana, misa centrifuge, vhura chivharo, uye gadzirisa zvekare zvibatiso zvemuenzaniso kana wedzera kana kubvisa masimbi ekuyera sezvinodiwa.

7. Dzokorora nhanho 5 uye 6 kusvikira centrifuge ichifamba zvakanaka pasina kuzununguka kana kuzununguka.

Izvo zvakakosha kuti uteedzere mirairo chaiyo yeimwe yako centrifuge uye rotor rudzi kuti uve nechokwadi chekuenzanisa kwakaringana. Pamusoro pezvo, kugadzirisa nguva dzose uye kuenzanisa kwe centrifuge kunogona kubatsira kuve nechokwadi chekuenderana uye kwakaringana mhedzisiro.

1. Kumhanyisa: Kushandiswa kwemutengi uye rudzi rwemasamples avanenge vachishanda nawo acharaira kumhanya kunodiwa kwecentrifuge. Marotor akasiyana akakodzera kumhanya kwakasiyana, saka zvakakosha kubvunza mutengi kuti yakakura sei yekumhanya yavanoda pakuparadzanisa samples.

2. Kugona uye uwandu: Saizi yemutengi saizi uye zvinodiwa zvemukati zvinofanirwa kutariswa. Ingani milliliters yemuenzaniso (centrifuge tube) mutengi anoda kuparadzanisa, uye yakawanda sei sampuli imwe neimwe inotarisirwa kuparadzaniswa panguva imwe chete zvinhu zvinokosha pakusarudza centrifuge yakakodzera.

3. Kudzora tembiricha: Mamwe sampuli anoda chaiyo tembiricha kudzora panguva centrifugation process, saka zvakakosha kufunga kuti centrifuge inodzorwa tembiricha kana kwete. Kana mutengi achida kupatsanura sampuli pane yakatarwa tembiricha, ivo vanozoda kusarudza tembiricha inodzorwa centrifuge.

4. Kudzora sisitimu: Iyo yekudzora sisitimu ye centrifuge inofanirwa kutariswa, kunyanya kana mutengi achida mamwe mabasa akadai sekuzivikanwa kwerotor, chengetedzo yekuvhara, kana kutadza kuratidza.

5. Noise level: Kana iyo centrifuge ichizoshandiswa munzvimbo yerabhoritari nemimwe midziyo kana vashandi, zvakakosha kufunga nezve ruzha rwepakati uye kusarudza modhi ine ruzha rusina ruzha kuchengetedza nzvimbo yekushanda yakagadzikana.

6. Zvishandiso: Zvimwe zviedzo zvinoda machubhu ecentrifuge akakosha kana midziyo yekuenzanisira, saka zvakakosha kuti titarise kuwanikwa kwezvishandiso zvinodiwa pakukurudzira centrifuge kune vatengi.

Nekutarisisa zvinhu izvi uye nekunzwisisa zvinodiwa nemutengi, unogona kuita kurudziro ine ruzivo yeiyo chaiyo centrifuge yerabhoritari yavo.

Zvimwe zvezvishandiso zvinodiwa zvecentrifuge zvinosanganisira:

1. Rotor: Rotor inhengo yecentrifuge inobata machubhu kana midziyo inenge ichirukwa. Zvakakosha kusarudza rotor inopindirana nemachubhu kana midziyo yauchange uchishandisa.

2. Adapters: Adapters anodiwa pakushandisa machubhu kana midziyo yehukuru hwakasiyana pane iyo rotor yakagadzirirwa.

3. Centrifuge tubes: Mhando dzakasiyana dze centrifuge tubes dziripo kune marudzi akasiyana-siyana emasampuli, akadai se conical pasi, pasi pasi, kana flat flat bottom tubes. Zvakakosha kusarudza rudzi rwakakodzera rwechubhu kune yako chaiyo application.

4. Tube sleeves: Tube sleeves inodiwa pakuisa centrifuging mamwe marudzi emasampuli, akadai sechepfu kana radioactive samples, kupa imwezve chengetedzo.

5. Mabhegi eropa: Mabhegi eropa zvigaba zvine hunyanzvi zvinoshandiswa kupinza sampuli dzeropa.

6. Temperature control accessories: Mamwe macentrifuge ane tembiricha inokwanisa, uye zvishongedzo zvakaita semarotor anodzorwa netembiricha kana mabhuroko emuenzaniso anogona kudikanwa kune mamwe maapplication.

7. Gas-tight adapters: Aya ma-adapters anodiwa kana achishanda nemasampuli asina kusimba, sezvo achidzivirira kubuda kana kusvibiswa kwemuenzaniso panguva ye centrifugation.

Paunenge uchisarudza zvishandiso zvecentrifuge yako, iva nechokwadi chekusarudza izvo zvinoenderana nemhando yako chaiyo ye centrifuge uye zvaunoda kuedza.

Iyo shoma nzvimbo inodiwa kuchengetedza feni kumashure kwe centrifuge zvinoenderana nehukuru uye dhizaini yefani. Kazhinji, kumwe kubvumidzwa kunofanirwa kusiiwa kwakatenderedza fan kuti ibvumire kuyerera kwemhepo, pamwe nekuidzivirira kubva kune chero kukuvara kunogona kuitika.

Kudengenyeka kwakanyanya kunogona kuitika kana rotor uye rusvingo rwemukati rwebhokisi re centrifuge risina kunyatsoenderana, kana rotor yakawandisa, kana kuti chiyero che rotor chadzimwa. Kuti udzivise izvi, mushandisi anofanirwa kuve nechokwadi chekuti rotor uye ndiro yakanyatsoenderana, kuti rotor haina kuremerwa, uye kuti rotor yakanyatsoenderana.

Musiyano mukuru pakati pepamusoro uye yakaderera kumhanya rotors ye centrifuges ndiyo yavo yakanyanya kutenderera kumhanya. High-speed rotor inogona kusvika pakumhanya kunosvika 100,000 revolutions paminiti (rpm), ukuwo marotor akadzika-speed anowanzo pfuura paanenge 10,000 rpm. Iyo yakanyanya kumhanya iyo yakachinjika rotor inogona kutsungirira zvinoenderana nezvakawanda zvinhu, senge saizi yayo, zvinhu, uye dhizaini. Nekudaro, marotor mazhinji akachinjika anogona kumira kumhanya kusvika ku30,000 rpm pasina kukuvadzwa kukuru kana kutadza. Zvakakosha kugara uchitevera kurudziro yemugadziri uye nhungamiro dzekuchengetedza paunenge uchishanda centrifuge kudzivirira kukuvara kana kukuvadzwa kwemidziyo.

Iyo centrifuge rotor ndiyo inotenderera ye centrifuge iyo sampu inoiswa kuti iparadzane.

Kune marudzi akasiyana emarotor anowanikwa kune chaiwo maapplication, asi zvikamu zveyakajairika rotor uye mabasa avanoita ndeaya:

1. Muviri: Muviri we rotor ndicho chimiro chikuru chinosungirirwa kumotokari ye centrifuge uye inobata zvimwe zvikamu panzvimbo.

2. Bucket: Mugomo wega wega kamudziyo kadiki kanochengeta samuenzaniso kuti iparadzaniswe. Mabhaketi anogona kuuya muhukuru hwakasiyana uye zvinhu zvinoenderana nechaiyo application.

3. Chivharo: Chivharo che rotor ndicho chinovhara bhakiti kuchengetedza sampuli panzvimbo panguva ye centrifugation.

4. Adapter: Adapter chinhu chinokwana mubhaketi uye chinoita kuti rotor igare machubhu ehukuru hwakasiyana kana maumbirwo.

5. O-ring: O-ring igasket rinogadzira chisimbiso pakati pechivharo nebhaketi. Inodzivirira kubuda uye kusvibiswa kwemuenzaniso panguva ye centrifugation.

6. Tube cushions: Tube cushions inoiswa mukati mebhakiti kana adapta kuti ichengetedze sampu uye kudzivirira kuti irege kukuvara panguva yecentrifugation.

7. Spindle: The spindle ndiyo chikamu chepakati che rotor iyo inobata zvinhu zvose pamwe chete uye inopindirana pakati pe rotor uye centrifuge motor.

Pakazara, izvi ndizvo zvikamu zvekutanga zve centrifuge rotor. Zvisinei, zvikamu uye mabasa azvo zvinogona kusiyana zvichienderana nerudzi uye kushandiswa kwe centrifuge, uye mamwe ma rotor angave ane zvimwe zvikamu zvakadai semaoko kana zvindori kuti zvigadzirire masampuli makuru.

Centrifuge mudziyo unoshandisa simba repakati kupatsanura zvinhu zvakasiyana mumvura kana musanganiswa.

Iyo ine zvikamu zvakati wandei zvakakosha, chimwe nechimwe chinoita basa chairo:

1. Motokari: Ichi ndicho chikamu chinopa simba centrifuge, ichipa inodiwa inotenderera simba.

2. Rotor: Ichi ndicho chikamu checentrifuge inobata sampuli kuti iparadzaniswe. Inotenderera pamwe chete nemota, ichigadzira iyo centripetal acceleration inodiwa pakuparadzanisa maitiro.

3. Tube Holders: Aya ndiwo maslots ayo emuenzaniso machubhu akaiswa mu rotor, nhamba uye saizi yayo inosiyana kubva ku centrifuge kusvika ku centrifuge zvichienderana nezvavanoda.

4. Chivharo: Chivharo chinogadzira chisimbiso chisina mhepo pamusoro pe rotor uye chinodzivirira zviri mukati kubva pakubhururuka kunze kana centrifuge iri kushanda.

5. Control Panel: Ichi ndicho chikamu che centrifuge chinobvumira mushandisi kutarisa uye kugadzirisa zvirongwa. Inosanganisira akasiyana mabhatani uye mapfundo ekudzora kumhanya, nguva, uye tembiricha ye centrifugation.

6. Brake: Kana nguva inodiwa ye centrifugation yapera, mabhureki anozodzikisa rotor kubva pahupamhi hwevelocity iyo yasvika. Pakazara, kuvakwa uye dhizaini ye centrifuge inogona kusiyanisa zvichienderana neiyo chaiyo application, asi zvakataurwa pamusoro apa ndizvo zvakakosha zveiyo centrifuge.

Zvakakosha kuziva chikonzero chemheremhere uye kugadzirisa nyaya yacho nokukurumidza kudzivirira chero kukanganisa kunogona kuitika kune centrifuge kana zviri mukati maro.

Panogona kunge paine zvinhu zvakati wandei zvinoita kuti centrifuge ive neruzha. Zvimwe zvezvikonzero zvinobvira zvinosanganisira:

1. Kusaenzana: Kana mutoro uri mucentrifuge usina kugoverwa zvakaenzana, unogona kuita kuti centrifuge izununguke uye iite ruzha.

2. Mabheji akasakara: Nokufamba kwenguva, mabheji ari mucentrifuge anogona kupera uye anoita kuti rotor izununguke, zvichikonzera ruzha.

3. Zvikamu zvisina kusimba: Zvikamu zvisina kusununguka kana kukuvadzwa, semabhandi, mabhaudhi, kana screws, zvinogona kuita kuti centrifuge iite ruzha uye kuita ruzha.

4. Kuremedza: Kumhanyisa centrifuge nekurema kwakanyanya kunogona kuita kuti iwedzere uye kuita ruzha.

5. Kusashandisa Zvisizvo: Kana iyo centrifuge isingashandisi maererano nemirairo yemugadziri, inogona kutungamirira kune ruzha.

6. Kuchembera: Mamodheru ekare emacentrifuges anogona kuburitsa ruzha rwakanyanya nekuda kwekupfeka uye kubvaruka nekufamba kwenguva.